一、基本信息

郁慧丽，博士，副教授，硕士生导师

电子邮件：huiliyu@hubu.edu.cn

研究领域：微生物次级代谢产物的生物合成，细胞色素P450酶挖掘，酶工程及绿色生物制造

二、教育背景

2013.10-2018.10德国马尔堡大学，药学系药物生物与生物技术研究所，博士

2010.9-2013.6山东大学，微生物技术国家重点实验室，硕士

2006.9-2010.6山东大学，williamhill威廉希尔，学士

三、工作经历

2021.10-至今williamhill官网，williamhill威廉希尔，副教授

2020.12-2021.10williamhill官网，williamhill威廉希尔，讲师

2018.11-2019.11德国马尔堡大学，药物生物与生物技术研究所，研究助理

四、主持科研项目

(1)细胞色素P450催化合成二酮哌嗪二聚体的底物特异性分子机制解析和理性改造 国家自然科学基金青年项目2025.1-2027.12，30万元，在研，主持

(2)催化高附加值化合物合成的P450酶元件表征 上海智峪生科科技有限公司2023.12-2028.12，27万元，在研，主持

(3)生物合成α-羟基脂肪酸工艺的开发 上海云洛生物技术有限公司2023.3-2024.3，10万元，结题，主持

(4)轻工业用关键氧化还原酶的分子设计与高效生产，国家重点研发计划子课题2021.7-2024.6，60万，结题，主持

(5)Streptomyces sp. 70中新颖环二肽合酶相关P450酶挖掘、功能及催化机制研究，湖北省教育厅，2021.1-2023.12，2万元，结题，主持

(6)人工改造P450生产香精香料，williamhill官网校基金，2020.12-2021.12，2万元，结题，主持

五、代表性论文（#共同第一作者*通讯作者）

1.Meng Dai#, Xiaojuan Yu#, Xiaogang Peng#, Zhiyong Guo*,Huili Yu*, Aitao Li Modulating regioselectivity of CYP107J3-catalyzed isophorone hydroxylation by disrupting the hydrophobic balance of the substrate binding pocket, Green Synthesis and Catalysis, 2024,https://doi.org/10.1016/j.gresc.2024.05.007

2.Huili Yu#, Xiaodong Zhang#, Carlos G Acevedo-Rocha, Aitao Li*, Manfred T Reetz* Protein engineering using mutability landscapes: Controlling site-selectivity of P450-catalyzed steroid hydroxylation, Methods in enzymology, 2023, 693, 191-229.

3.郁慧丽，李爱涛*细胞色素P450酶在香精香料绿色生物合成中的应用[J].生物技术通报，2023, 39 (4), 24-37.

4.Ruiwen Hu#, Anjie Gong#, Langxing Liao#, Yan‐Xin Zheng#, Xin Liu, Peng-Wu, Fushuai Li,Huili Yu, Jing Zhao, Long‐Wu Ye*, Binju Wang*, Aitao Li* Biocatalytic aminohydroxylation of styrenes for efficient synthesis of enantiopure β-amino alcohols. Chinese Journal of Catalysis, 2023, 44, 171–178.

5.Huili Yu; Xiulan Xie; Shu-Ming Li*; Coupling of cyclo-l-Trp-l-Trp with Hypoxanthine Increases the Structure Diversity of Guanitrypmycins, Organic Letters, 2019, 21(22): 9104-9108.

6.Huili Yu; Shu-Ming Li*; Two Cytochrome P450 Enzymes from Streptomyces sp. NRRL S-1868 Catalyze Distinct Dimerization of Tryptophan-Containing Cyclodipeptides, Organic Letters, 2019, 21(17): 7094-7098.

7.Huili Yu; Xiulan Xie; Shu-Ming Li*; Coupling of Guanine with cyclo-l-Trp-l-Trp Mediated by a Cytochrome P450 Homologue from Streptomyces purpureus, Organic Letters, 2018, 20(16): 4921-4925.

8.Jing Liu#;Huili Yu#; Shu-Ming Li*; Expanding tryptophan-containing cyclodipeptide synthase spectrum by identification of nine members from Streptomyces strains, Applied Microbiology and Biotechnology, 2018, 102(10):4435-4444.

9.Huili Yu; Mike Liebhold; Xiulan Xie; Shu-Ming Li*; Tyrosine O-prenyltransferases TyrPT and SirD displaying similar behavior toward unnatural alkyl or benzyl diphosphate as their natural prenyl donor dimethylallyl diphosphate, Applied Microbiology and Biotechnology, 2015, 99(17): 7115-7124.

10.Huili Yu#; Haoran Li#; Dongfang Gao; Cuijuan Gao; Qingsheng Qi*. Secretory production of antimicrobial peptides in Escherichia coli using the catalytic domain of a cellulase as fusion partner, 2015, Journal of Biotechnology, 214, 77-82.

11.Dongfang Gao; Shengjun Wang; Haoran Li;Huili Yu; Qingsheng Qi*. Identification of a heterologous cellulase and its N-terminus that can guide recombinant proteins out of Escherichia coli, 2015, Microbial Cell Factories, 14, 49.

12.Shengjun Wang; Chuanliang Ma;Huili Yu; Changgan Huang; Qingsheng Qi*. Development of a method for the efficient release of N-glycans from glycoproteins generating native deglycosylated proteins, 2012, Enzyme and Microbial Technology, 51, 139-142.

13.王圣钧,郁慧丽,翟琳,王倚,梁泉峰,祁庆生*.人GLP -1- IgG Fc融合蛋白在毕赤酵母中的高效表达[J].生物技术, 2011, 21(4), 29-33.

六、荣誉及奖励

2021 湖北省“楚天学子”